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Remote Desktop Fails as soon as a RDS Collection is Created

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Abbreviated Problem: 

As soon as I create a RDS application collection I am unable to use any remote desktop services.  Reboot has no effect. Security Layer is set to "Negotiate" and Encryption Level is "client compatible".  Prior to creating the collection standard RDP works; after it is created, however, RDP (including remote apps) fail to connect with the error :



Likewise, Immediately upon deleting the collection, Remote Desktop begins working again.

-This is a quick setup RDS deployment that exists only on one virtual Server 2016 instance.

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Additional info:

The collection was working as intended for several months but I discovered that the above error would occur for all users and devices after a reboot of the RDS server or after a restart of the remote desktop services on the RDS server.  Heeding the advice of a RDS forum I deleted the application collection and thought my troubles were behind me once remote desktop began to work again.. wrong.. As soon as a new RDS collection is created the error returns for all connections.  I initially created the collection over an RDP connection and thought that might be the cause but the issue remains even when using a vmware console while creating the collection.  

Obviously my RDP ports are open from the client computers to the RDS since RDP works after the collection is deleted -windows firewall is also off.  

Hoping someone with more expertise and experience with RDS can help here.  






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